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anti penta his primary antibody mouse  (Qiagen)


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  • 97

    Structured Review

    Qiagen anti penta his primary antibody mouse
    Anti Penta His Primary Antibody Mouse, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1658 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+primary+antibody+%CE%B1+his/Penta+His+Antibody%2C+BSA-free/pmc13174244-3-0-5
    Average 97 stars, based on 1658 article reviews
    anti penta his primary antibody mouse - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: The molecular chaperone DNAJB6 provides surveillance of FG-Nups and is required for interphase nuclear pore complex biogenesis
    Article Snippet: .. After blocking for 1 hour in 2.5% BSA (Albumine bovine serum, Acros Organics 268131000, #10450141) in PBS with 0.1% Tween20 (MP BiomedicalsTM, MP1TWEEN201), the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C, washed three times with PBS-T, incubated with anti-mouse secondary antibody m-IgGk BP-HRP (1:5000, Santa Cruz Biotechnology, sc-516102) in 2.5% BSA in PBS-T for 1 hour at room temperature, and washed three times with PBS-T. Chemiluminescence was detected using ECL substrate (GE Healthcare, RPN2232) according to manufacturer’s instructions on a ChemiDoc Touch Imaging System (Bio-Rad). ..

    Membrane:

    Article Title: The molecular chaperone DNAJB6 provides surveillance of FG-Nups and is required for interphase nuclear pore complex biogenesis
    Article Snippet: .. After blocking for 1 hour in 2.5% BSA (Albumine bovine serum, Acros Organics 268131000, #10450141) in PBS with 0.1% Tween20 (MP BiomedicalsTM, MP1TWEEN201), the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C, washed three times with PBS-T, incubated with anti-mouse secondary antibody m-IgGk BP-HRP (1:5000, Santa Cruz Biotechnology, sc-516102) in 2.5% BSA in PBS-T for 1 hour at room temperature, and washed three times with PBS-T. Chemiluminescence was detected using ECL substrate (GE Healthcare, RPN2232) according to manufacturer’s instructions on a ChemiDoc Touch Imaging System (Bio-Rad). ..

    Incubation:

    Article Title: The molecular chaperone DNAJB6 provides surveillance of FG-Nups and is required for interphase nuclear pore complex biogenesis
    Article Snippet: .. After blocking for 1 hour in 2.5% BSA (Albumine bovine serum, Acros Organics 268131000, #10450141) in PBS with 0.1% Tween20 (MP BiomedicalsTM, MP1TWEEN201), the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C, washed three times with PBS-T, incubated with anti-mouse secondary antibody m-IgGk BP-HRP (1:5000, Santa Cruz Biotechnology, sc-516102) in 2.5% BSA in PBS-T for 1 hour at room temperature, and washed three times with PBS-T. Chemiluminescence was detected using ECL substrate (GE Healthcare, RPN2232) according to manufacturer’s instructions on a ChemiDoc Touch Imaging System (Bio-Rad). ..

    Imaging:

    Article Title: The molecular chaperone DNAJB6 provides surveillance of FG-Nups and is required for interphase nuclear pore complex biogenesis
    Article Snippet: .. After blocking for 1 hour in 2.5% BSA (Albumine bovine serum, Acros Organics 268131000, #10450141) in PBS with 0.1% Tween20 (MP BiomedicalsTM, MP1TWEEN201), the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C, washed three times with PBS-T, incubated with anti-mouse secondary antibody m-IgGk BP-HRP (1:5000, Santa Cruz Biotechnology, sc-516102) in 2.5% BSA in PBS-T for 1 hour at room temperature, and washed three times with PBS-T. Chemiluminescence was detected using ECL substrate (GE Healthcare, RPN2232) according to manufacturer’s instructions on a ChemiDoc Touch Imaging System (Bio-Rad). ..



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